...suppresses neuronal transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to ...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert...immune genes into pXPR_053. They expressed this construct in bone marrow stem cells from Cas9-expressing...

...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...  A second application for CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction... BirA, and 3) one or more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP...

...microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes (the earlier...earlier article also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4)...100x variants. But those super-high efficiency constructs are great for in vitro biocatalytic applications...

...assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can...gene-of-interest, all the way to the complex multi-TU constructs that compose genetic circuits and metabolic pathways...MoClo is commonly used to design TUs and multi-TU constructs for synthetic biology applications in bacteria...individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016). The variety of constitutive...multiple unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for...is geared towards cloning multi-TU expression constructs (Martella et al., 2017). If you can dream up ...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...

...with biotin as an affinity tag. This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9...

...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...efficiency was not affected by CellTagging. By constructing lineage trees and comparing them with the gene...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101...

...increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...

...was rapidly able to support sharing of CRISPR constructs and information across the globe.  “Read the ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

...in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these...on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! ...

...promoters is commonly employed in lentiviral and retroviral constructs and we will discuss these in a future...

...selectable marker. This vector can express your construct in target cells transiently or constitutively ...Some customization is required, but many viral constructs have already been built and are available on ... embryonic stem cells. Viral integration of a construct is also reliably higher than traditional transfection...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

...the design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...that particles are not sprayed into the air. For lentivirus and other BSL-2 materials, you’ll need to use...for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which...

...justifying each single element used to build the construct and explain why they selected a certain target...has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

...however, suffer from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome...be directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...