...viral production and distribution, this project involved every Addgenie in the company. Our Finance, Business...continues to make improvements to our website and inventory management systems to make way in our systems ...Maintaining our excellent service and accuracy involves caring and careful Addgenies, but also a lot of...Software and IT Teams made major upgrades to our inventory management systems to enable efficient, tracked...

...to do that we’ve had to navigate the challenges involved in plant synthetic biology. Challenge #1: Public...unique metabolic processes of plants are being investigated on limited funding and time. While institutions... standing behind it. Our color changing flower involves two basic components: the pigments (anthocyanins...research for decades, and the biochemical pathways involved in central anthocyanin metabolism are well understood...

...using the kit to target a large number of genes involved in cardiovascular and metabolic diseases. We are...Addgene: Tell me about the history. How had investigators done this kind of work before? Musunuru: In ...Unfortunately, it is time consuming and difficult for investigators without a lot of expertise to create zinc finger...and puts it in the hands of pretty much all investigators. Our kit makes it extremely simple to design...

... of genes involved in the execution of a specific regulatory regime (such as genes involved in metabolism...unknown. Together, NRs govern expression of genes involved in a broad range of reproductive, developmental...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...

...networking with your local education community. Get involved in education advocacy non-profits like Phipps ...science projects Lastly, if you'd like to directly involve the public in your research projects, you can set...genomes requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload...project. Science communication opens doors Getting involved in science communication has been the most rewarding...

...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience... is now studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello...presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families... natural role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile...

...public to access basic equipment and to become involved in developing tools and solutions to research ...makerspace is. The computer scientists can get involved in modelling, the electronics folks can build ... top of that model, and the biologists can get involved a in variety of aspects from the molecular biology... feel free to contact your local space and get involved! Derek Jacoby is a PhD student at the University...

...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...applications. But how does one invent a tool for applications that don’t even exist yet? Andrew York, Maria...applications are still yet to be demonstrated (or invented), its behavior has already been successfully reproduced... chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts to explore...

...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

...step and mix the samples well by vortexing or inversion. Created with BioRender.com.   When choosing...curve, change pipette tips and mix thoroughly by inversion or vortexing between each step of the series. ... dynamic range of the curve, then the assay is invalid. If the unknown sample’s measurement is too low...increase the accuracy of the data. For assays involving sigmoidal curves, manufacturers often recommend...

...researching, winning, and effectively managing grants involves much more than writing. In fact, many grant applications...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...organization in a position that required some involvement in the grants process and managing programs that...

...experimental hurdles I would encounter. This was invaluable to me as a bench scientist working at an institution...available. Even more valuable, however, was when she invited me to lunch for a “tweet-up” with other folks at...not participate but provided moral support was invaluable.  Mixing metaphors and scientists - Culturing...participants feel like their mentors weren’t as invested in how their meetings went. While this was expressed...

...months, in fact. In the Lee-Parsons lab, we are investigating how the production of cancer therapeutic drugs...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...transient methods are usually the first choice for investigating gene function, as these experiments can be done...species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ages Usually younger...

...high-quality maps and annotations without us having to invest too much of our internal software development resources...zone As part of my position at Addgene I am now involved in Strategic Partnership Management, a very interesting...for discussions and potential collaborations. We invited experts from other universities and maintained ...ways to maintain a relationship - many of them involve personal meetings and common interest so naturally...

...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...

...experiment-based investigation of a hypothesis: proper controls! For every experiment, an investigator needs a ...an experiment lacking the proper controls are invariably inconclusive and unreliable. Proper controls ...tenet of responsible scientific inquiry and investigation. References: 1. The problem of pseudoreplication...

...hippocampus and the prefrontal cortex brain regions is involved in the formation of episodic memory, a special...for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids...reach the affected part of the brain in a less invasive manner . For example, in neuromuscular diseases...direct intracerebral application, which is more invasive and has a much higher risk of complications. ...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...Integrins are essential cell surface receptors involved in complex signaling pathways and the linkage ...researchers can measure this conformational change to investigate the role of specific integrins in cellular processes...subunit. Using these affinity reagents, the investigators inhibited other RGD-binding integrins, individually...for cell adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 ...

... biology. This type of cell labeling technique involves the introduction of a transgene construct containing...is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation...variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes aligned head-to-head...Mouse Model of Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): ...

...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...