Skip to main content
Addgene
Showing: 41 - 60 of 74 results

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ... zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors ...

  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic ...

  7. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...

  10. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... kb. This system is transfected into cells as a donor plasmid containing the transposon, along with a ...helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest flanked...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...frequencies and relies on the efficient delivery of donor DNA to facilitate repair. Since the development ...
Showing: 41 - 60 of 74 results