Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
Type
Blog Post
Published
April 5, 2018, 12:42 p.m.
...how you can make CRISPR mouse model generation easi-er!
Generating mice before CRISPR
The process of generating...crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection...Addgene.org
Check out our CRISPR Guide
Find Plasmids for Cas9 Purification
Find CRISPR Plasmids for Mammalian...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor... generating knock-in mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired...