...guide that directs cleavage right before the stop codon!   Design your repair template with 55-100 base ...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort...

... active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac ...

... to the use of exon skipping, alternative start codons, and/or the phenomenon of transcriptional adaptation...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ...

...lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol...

...synaptic glutamate response. They generated variants optimized for different applications (v82 has higher affinity...indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized for two-photon imaging. They use a unique and ...multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors...variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a significant...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators ...