.... The two remaining plasmids in the kit have the rest of the protein in place including FokI endonuclease...new gene variants associated with conditions of interest, then studying those variants in model systems...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...

...their precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes...the target DNA strand, makes few contacts with the rest of Cas9 and is highly flexible; it can rotate about...

...target protein remains bound to the column while the rest of the proteins are eluted. Then, the antibodies...isolate antibodies only recognizing your protein of interest: affinity-purified antibodies are isolated from...

...of R, is capped, and proceeds through U5 and the rest of the provirus. 3’ LTR Terminates transcription...plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by long terminal...

.... We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene chose this...

...Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed ...Zhang lab is used to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top ...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

... translate that valuable information back to the rest of my team in Oxford (UK) to help solve those problems...realized it was a great fit for my skillset and interests. As such, I want to be able to share more about...the North America region. She is particularly interested in DNA sequencing tech, and the intersection ...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...

...preparation should be doing your best to be well-rested and alert the day of the procedure. Mice move much...

...not happen in a vacuum that is isolated from the rest of society or devoid of social context. This “general...process. My role at pgEd I have always been very interested in the societal aspects of science, even while...science communication or policy requires broad interest in issues at the interface of science and society...narrowly-defined research questions in our field of interest. We often communicate in very technical and jargon-filled...jobs and those of their families. Having a broad interest and awareness of issues outside of your field ...them. Researchers currently in academia who are interested in such careers should take advantage of their...Genetics Education Project (pgEd). He is broadly interested in the legal, ethical and policy issues at the...

...formats helps these statements stand out from the rest of the text. Specific aims The Specific Aims section...Develop a research plan Most grant applications restrict the space available to describe the proposed work...

...paired with dimmer dyes so they don’t “wash out” the rest of the panel and create spreading error. Conversely...

...locus, provided the target is unique compared to the rest of the genome and located immediately 5’ to a protospacer... What genes are important in your phenotype of interest? Many scientists study diseases for which the ...drug-resistance. Mutant cells are treated with a drug of interest and gRNA distribution is analyzed in the drug-...can help you learn a lot about your phenotype of interest. For more detailed information on CRISPR screens...

...our live examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind...

...is the right way?  First of all, I want to put to rest a common misconception. I find personal bias often...If you're interested in sharing your science, one of the big questions you'll be faced with is "how?"...be able to access your content.  What are you interested in? Math, sarcasm, romance, and spacecraft...can guarantee you that either resource or time restrictions (or both!) will be a major limiting factor at... to reach people who are not already actively interested in scicomm. Difficult to do in-depth storytelling...

...minutes of the two hour time slot, so she can take the rest of the time to collect her data. Perhaps you both...

...protein-coding genes, and you might be wondering what the rest of our genome could possibly be made up of. The ...options. By carefully considering your ncRNA of interest, you should be able to devise an experiment that...

...CRISPR tools were committed to sharing them with the rest of the community. They did this by depositing them...

...genome scale, it's going to be tricky to tame the rest of a cell. The BIOFAB plasmids represent the first...

...sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing and...This score is a useful measure for those who are interested in understanding how many of the contributing...