...considered if size constraints are an issue. Adenovirus Adenovirus has a linear, double stranded DNA genome...serotypes of human-infecting adenovirus have been identified so far! Adenovirus generally has high immunogenicity...viruses are roomy: some adenoviruses pack up to ~35 kb of space for cargo! Adenovirus is an effective option...used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...mammals, and even the avian population. Some adenoviruses are known to cause respiratory disease across... the other viruses discussed in this article, adenovirus has the highest maximum titer, a plus for production... genomic integration. While the expression of adenovirus cargo is high, it’s generally not as long lasting...

...’s lab in Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc...research. When she came to Novartis, she was happily surprised that “Novartis was actually really research...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new tool that allows ... and says that she decided to do a post-doc at Novartis because she wanted first-hand experience in industry...investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ...developing more potent ABL1 allosteric inhibitors at Novartis. The ClonTracer library has been distributed all...

...However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable...Creativity is required to develop new projects and test novel approaches, a necessary skill set for any scientist...ll delve into strategies you can use to foster innovation. If you identify as a non-creative or if you ... by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself a block of...starting point, the ideas generated were hardly novel. When told to use a roller skate as a starting point...but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box concept and ...As a result, the product team is an incredibly innovative group. Our entire company benefits from their...

...scientific teams at the Novartis Institutes for BioMedical Research to develop novel biologics for the treatment...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting...Crystal's educational background and position at Novartis. Chapter 2: What's a lab group in industry? The...empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself for success by having... The types of science that Crystal works on at Novartis, how she's learned to prioritize projects, and...listings. Mobility between different positions at Novartis. Chapter 7: How Crystal developed her science ...

...fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic variant of...AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex,...Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to ...observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector...genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods...characteristics they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1...technique using small scaffold proteins to identify novel binding proteins that bind to either the light- ...

...this post: Improved voltage indicator: JEDI-2P Novel class of light-gated potassium channelrhodopsins...https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of light-gated potassium channelrhodopsins...labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that HcKCR1 and...photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs represent a long-...performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural...

...mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). While each...higher end. Adeno and adeno-associated viruses Adenoviruses infect non-dividing cells but they do not permanently...integration causing mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (...non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which means they...cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional... adenovirus...

...Dumas, Lercher, Spicer, & Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher...engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the UAG codon in...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...experimental design lends itself to creativity and innovation, valuable commodities in any NIH grant application... understanding of your protein that can lead to novel insights about its biology and its behavior. See...in Boston, MA. His interests include developing novel fluorescent tools for labeling reagents and structurally... of ryanodine receptor type 1 structure using a novel site-specific labeling method." PloS one 4.10 (2009...

...did we get here in the first place? How CRISPR innovation affected software development CRISPR, like the...it, offers an interesting view into how rapid innovation affects the research community. I’ve likened ...That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata), and software development has caught...separate sgRNA design tools into database and de novo solutions. Database tools allow us to view and get...community in order to succeed. With respect to de novo sgRNA design, I again separate it into two categories...future of CRISPR software is promising. Rapid innovation in this space has put us in a good position to...

...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...verify excision didn’t introduce mutations Adenovirus Non-integrating Low Medium Generates transgene-free...polycistronic transgene or with four different adenoviruses, each expressing one factor. These vectors don...less likely to cause insertional mutagenesis, adenoviral vectors are considered a safer way to express...Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated...

...replication when copy number in the cell falls too low (Novcik, 1987).  Antisense RNAs encoded on the plasmid ...plasmid for replication or partitioning machinery (Novick, 1987). In cases where one plasmid outcompetes ...incompatibility to combat antimicrobial resistance. Using novel plasmids based partly on plasmid incompatibility.../j.1472-765x.2008.02426.x Pubmed PMID: 19241516 Novick RP (1987) Plasmid incompatibility. Microbiology... 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility of plant...

...animal testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids... writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s first genetic...against a genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie version (2016) stars...editors which can help accelerate the engineering of novel base editor proteins. She is particularly interested...Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ (2011) Targeted Genome Editing Across...

... changed its name from Novophage to Sample 6. To learn more about Novophage's successful transformation...Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however, the...firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics...

.... https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent enzyme biosensors provide direct, specific...optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating a SL-insensitive...parasitic Striga hermonthica SL receptor HTL7. This novel type of biosensors could be used in high throughput...As a result, agricultural applications of these novel biosensors could have a major economic impact.   ...

...of the company.  Ultragenyx’s goal is to develop novel products for the treatment of rare and ultra-rare...was the Head of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development...are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding ...built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says. “The continuous...

..., founding bioethicist of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell...forward” is the June 2012 report on the ethics of “Novel techniques for the prevention of mitochondrial DNA...Nuffield Council began their research into both novel and previously established ethical issues raised...industry to inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic...

...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...become blocked, resulting in reduced blood flow. Novel treatment options, as well as non-pharmacological...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated CRISPR labeling...minutes and red foci formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted ...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein...

...have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles... AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped... requires co-infection of helper viruses like adenovirus for productive infection. AAV vectors are engineered... have slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and...

...Commun. 2017. PubMed PMID: 28646204. soCoChR: A novel opsin that enables single-cell optogenetics Article...system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal networks...developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with an absorption...absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation...