...paper and then another figure in another paper. A large fraction of readers cover the same ground again ...allows you to make meaningful collections from a large section of open access life sciences data. You can...

...his team created with the research community at large, "[Fluorescent proteins are] a whole new way of ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

... SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented...delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was...

...Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these two requirements...interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing...

...III-E, and then used this information to identify a large variety of related bacteria with similar Cas systems...fused integrase would then integrate into with a large DNA insert – or Programmable Addition via Site-specific...programmed for new genes. In comparison with other large insert techniques like HITI or HDR, which require... and adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic...

...into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...defects in the gene MYO7a, at ~7 kb this gene is too large to deliver via AAV.  Dual vectors strategies are... to regulate and optimize transgene levels, and large scale manufacturing of viral vectors to support ...cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered using this vector...

...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

... in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both...the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively low immune...lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for ...Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien...

...detection; however, those are just one (admittedly large) class of common fusion protein tags. Biochemists...; has inherent enterokinase cleavage site GST Large Protein 26 Purification and Stability Good for purification..., flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can improve...

...observed to occur more frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats...reads (<300 bp) which cannot distinguish between large repeat sequences within a plasmid, it cannot be ...sequencing. These longer reads are able to detect large repeat sequences within a plasmid and, therefore...DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12...just as well as monomers. However, due to their larger size, multimers may exhibit reduced transformation... & Sherratt, 1984; Summers et al., 1993). These larger plasmids are also maintained at lower copy numbers...monomeric version. However, undigested multimers are larger than monomers and will therefore run higher on ...

... that cellular mismatch repair contributed to a large number of unintended prime editing outcomes, including...combine prime editing with a recombinase to install large sequences (up to 10 kb) into the genome (Pandey ...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...J. S. (2023). Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using...

...overheating remains an issue, which can happen with large proteins, try lowering the voltage and using a longer...you know you loaded a good amount of sample of a large protein, you may be under-transferring.  Membrane...good indication that the p membrane pores are too large.  Transfer method   Wet transfers are more efficient...efficient, so if you’re struggling to transfer a large protein, consider using a wet transfer with a long... lot of antibodies. If you’re planning to use a large amount of a specific antibody, plan ahead and request...harsher stripping buffer Too little signal Large proteins: Incomplete transfer Check transfer with...proteins going completely through the membrane or larger proteins not transferring completely onto the membrane...

...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR...

...plasmids as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later...that plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify...

... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

...position as an Outreach Scientist where I spend a large amount of my time meeting with and talking to graduate...experience is key to my current position, I think it’s largely my work developing myself as a science communicator...

...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

...and to verify the quality of the prep. This was a large factor in Addgene’s decision to launch our viral...category, available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors...were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls”...

...guides directed towards that gene as there can be a large difference in guide potency. Thus, in cases where...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight...target effects are generally not regarded as being a large problem for Cas9 activators. This is believed to...makes it much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based...