CRISPR 101: Targeting RNA with Cas13a (C2c2)
Type
Blog Post
Published
Sept. 21, 2017, 2:07 p.m.
... using a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...