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  1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP is added to the wells. This... the amount of your protein of interest in the original sample. The competitive ELISA is best used for...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent protein...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...applications. Get More Cpf1 Background Here THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN ... What we still don't know about Cpf1 In their original paper characterizing Cpf1, Zetsche et al. suggested...

  5. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...enable us to measure these properties, and what fascinating experiments could push these tools even further... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... structure of a protein, based on its atomic coordinates. These wire models were useful in that they not...download a pdb file of the structure’s atomic coordinates, and “open” these structures with molecular viewing...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...affinity for specific cell and tissue types. The combination of serotypes and psuedotypes means you can customize...stably express Cas effectors in specific tissues, eliminating the need to deliver the Cas protein altogether...

  8. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent assay...each sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...exchange occurs through two sequential homologous recombination events: The plasmid carrying the desired genetic...activity. ShadowR can be used as a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa ...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...Scientific #1011-8410) is also placed in the hood. Finally, a sterile 2X DMEM solution (diluted from 10X and... backup replicates of each clone in case of contamination. We do not normally use antibiotics. Lastly,...

  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...opportunities available to me as a graduate student culminated in my founding a nonprofit called Central Indiana...skills, and meet new people. There were visiting seminar speakers each week, my department and graduate ...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... of two proteins from a single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes... Melina Fan...

  13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...actively use tools designed to help reduce or eliminate bias, just like the ones made available through.../s41586-022-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo,...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...possible, unlike methods involving homologous recombination. Additionally, these transposon also exhibit...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

  19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... cognate protein. In some cases this property eliminates the need for removing excess dye from the sample..., are photoactivatable, and function in vivo.* Final thoughts The ease of use and continual improvement...

  20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ..., such as knockout generation or homologous recombination.  However, experiments that require long-term... laboratory reagents, equipment, and expertise. Final thoughts The Cas9-gRNA RNP method provides scientists...
Showing: 561 - 580 of 763 results