...scientists to get out there and talk about their work. To tell others why that work is cool and why it really matters...seem all that interested in the details, work on telling it another way. You’ll know you are on the right...Plan to write regularly and, when you are ready, tell your friends or others in the field where to find... science writing, look into a AAAS Mass Media Fellowship or maybe an internship at a place like Science...

...devise new strategies to reproduce within a host cell. A few virus particles have learned to circumvent...deliver a highly specific, programmable platform well-suited for various innovative biological and translative...2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432...): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. ...

... GFP, enabling you to select cells with high GFP expression. These cells have the highest levels of Cas9... on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the desired...gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification. In this..., et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.” Scientific...

...2016). It’s also been well established that recombinant antibodies are excellent for reproducibility because...protocols for recombinant antibody production as well as antibody applications. In addition, basic and... can transform the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning...

...graduate student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology...untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...observing RUBY compared to other markers lends itself well to nondestructive selection. If RUBY was integrated...

...Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen...nanobodies to study retrograde transport from the cell surface. Their toolkit includes a set of plasmids...new technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist...an sgRNA expressed from a separate plasmid. When cells are treated with biotin–phenol and H2O2, APEX2 labels...

... useful and productive, forming a different constellation of interests and skills than what fits for applied...Queen” shows how punctuation can be wildly funny as well as financially critical. Those who work on the publishing...outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs at various...sciences, viewed through the lens of development, cell, and molecular biology. Beyond that, she is also...

...thermoplastic polymers that can work with all the bells and whistles. The fibers are made from a process...later! Next, additional components are added - the ‘bells and whistles’ – including microscale light emitting...of the stiffness the fibers would need to be, as well as other features necessary for implantation in ...and chemically excited a class of enteroendocrine cells within the intestines known as neuropods. They observed...

...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions... research; provide specific examples if possible Tell them about the Golden Stopwatch! Any organization...University  Princeton University  Purdue University Rockefeller University  Salk Institute for Biological Studies...agreement Technology Transfer: the exchange of intellectual property (as inventions, ideas, plasmids) among...

... be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In contrast...unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements. To...plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9...

...planning for your visa and or green card process well before addressing your career alternatives in order...the United States including green cards that are well-tailored to meet the needs of researchers. The categories...related skills”. Many career paths in academia as well as the non-profit sector are transferrable to industry...national importance; (2) The foreign national must be well positioned to advance the proposed endeavor; (3)...below. An international or national award for excellence in your field. Membership in a professional organization...

...CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton Learn How Plasmid...while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston University...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge...

...spend the afternoon lovingly tending to the stem cells I was using in an experiment, then spend the evening...evening listen to some bloke at the pub telling me that actually he drives better after a few drinks. A...it’s oddly fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years...Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., & Mandel, G. (2012). The polarizing...

... colleagues. When you attend a well-run meeting, note what worked well and ask the meeting leader for ...annoying interruptions to our “real” work. However, a well-run meeting can have quite the opposite effect. .... We’ve provided some tips above for what works well at Addgene, but meetings are never one-size-fits...

...expected to make sure the work gets done and done well. Involving your team effectively is now your new... in mind, you won’t be able to communicate these well to the task assignee and this is a recipe for bad...delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities, good ...Delegating" in the Harvard Business Review. Another excellent blog on delegation tips and practices can be found...

... be wondering how this all fits in with science. Well, I’ll give you an example. At my previous company... goes into software product management? I always tell people the best part of my job is getting to talk...communicate with customers, software developers, as well as team members in automation, sales, marketing,...help you be a valuable asset to the scientists as well as the developers. Lastly, you’re going to need ...

... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent... Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role..., Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions...

...idea from academia to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing...aligned in vision and outcomes. The team must work well together, have complementary skill sets, and “talk...were free and free to repair.” If the team works well, the idea is solid, and “the pitch is packaged to...

...you correct any language- and spelling mistakes. A manuscript that is well written looks more professional...submission, missing files or a manuscript full of spelling errors will not have a great chance of getting...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

...practical solutions.  My PhD prepared me remarkably well for this task. Good scientists learn how to communicate...to say “I’m going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different...different types of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create...It is never really done. But if you implement it well, here’s what can happen: More effective meetings...