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  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear....  Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually...

  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...need to keep paper records as well as electronic ones? It is important to know in which ways your data...functionalities such as: electronic signatures, electronic witnessing and audit trails. How does the ELN provider ...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of...

  8. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor molecules for homology...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ...

  11. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine amino acid...the fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active ...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of...

  15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...above 30% for a variety of targets in human cell lines, with an average indel frequency of only 1.1%. These...which is significantly more effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following...

  19. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday...
Showing: 541 - 560 of 738 results