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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 780 results for: ina

Showing: 541 - 560 of 780 results

  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate. This...you used? Share your tips below! This post was originally written by Jason Niehaus in June 2014 and was...

  2. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...supportive environment to build my management skills. Finally, developing emotional intelligence is vital. Learning...collaboration. The good news: It’s a journey, not a final choice Management is not a one-way path. As careers...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...identifying guide selection rules, and reducing recombination by finding acceptable variations in DR sequences... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...dissociation from the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently...pathways from ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples...

  5. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...website, as an instructive step-by-step guide.  Finally, when writing your alt text, use all of this information... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons ...

  8. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... very similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead... medium to allow RLuc8 to produce photons. The original nano-lantern emitted yellow-green light and exhibited...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate... only be expressed in cells that express cre recombinase. Typically, this feature is used to attain cell-specific...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...dCas9 fused to the Activation Induced Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful scientists ... graduate students in different regional and disciplinary communities gather at ComSciCon's local and ...

  15. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

  16. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...Networking - Building Relationships for Success" webinar with Joanne Kamens via ASCB Check out more Career...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

  20. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...
Showing: 541 - 560 of 780 results