...circumstances – including unpredictable loud noises and flashing lights - that sent average, control mice into ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

... Sequencing Resources: Tips for Using BLAST to Verify Plasmids Acknowledgements: Thank you BitesizeBio...on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing...

...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

...Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data showing that this...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology. Springer US, ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...

... Though you’ll want to predict this value using BLAST, you’ll need to experimentally verify once you’ve...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This... of the algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not...

...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...

...viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific...combinations to choose from for your experiments! Late last year, we hosted our first AAV Education and Development...of molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT...

...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

...These receptors are specific to isotype class or even subclass, and are part of the reason why different...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral...community.  Be the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review...

... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs... were partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...

...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...

...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target...

...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory...

...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...Looking for a bright, monomeric FP published in the last few years with an emission peak around 600nm?  Simply...

...Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our Stem Cell Pages ...gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting step in stem...