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Showing: 521 - 540 of 738 results

  1. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

  8. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  9. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

  10. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  11. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...protocols and examples of applications from across disciplines now available. In this post, we’ll review the...example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes take on a ...

  15. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation...

  16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...Japan, of course, it is optimized for living for Japanese, which is not trivial, and the grant situation...eager to interact, which I don't think is good for Japanese science. I really miss the intellectual environment...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly...

  18. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock...

  19. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...
Showing: 521 - 540 of 738 results