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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 780 results for: ina

Showing: 521 - 540 of 780 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...beamsplitter, and a 495/30 nm emission filter. This combination results in a 2.6-fold increase in signal relative...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging...

  2. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...will help get your experiments back on track.  One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored... in areas such as bacterial conjugation and recombination, replication and topology, and cloning and gene...

  6. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...critical three letters after your name (P-H-D).  Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we...

  7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now...elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...

  9. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded...

  12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly...

  16. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...clusters of individual cells along with color coded nominated reference cell types.  (Adapted from Joung et ...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document...
Showing: 521 - 540 of 780 results