...techniques comes in handy whether you are perusing classic papers or are brainstorming new technological innovations...blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

...to whole-body in small animals, such as mice (Matlashov et al., 2020). Enhanced miRFPs are monomeric and...NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al., 2020) derived from bacterial phytochrome...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from ...ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared ...

...ever watched any behind the scenes footage in the last several years, you can usually see a director sitting...the subject after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation....beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them....a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the office....

...and quality controlled plasmids and viral vectors. When working with a plasmid from Addgene, you can be...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility...Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45 - Reagent Development...

...finishing my MSc. The masters program was 6 months of classes and 6 months of hands-on lab work for a dissertation...would like to do. I had taken advanced virology classes in California and enjoyed them, so I applied for...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...

...Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous liquids. Working...Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting...responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more...Feeling Like an Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...acid) that reacts with biarsenical dyes such as FlAsH (green, based on fluorescein) or ReAsH (red, based... Tetracysteine (Cys4) 15 Biarsenical dyes (FlAsH, ReAsH) Few Easy SNAP-tag 182 Benzylguanine...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

...Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years, there's been a disturbing...great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science...Golden Hi, my name is Erin and I have spent the last 15 years training as an academic scientist. I’ve...

...going viral,” we also have some other exciting news. Last week, we launched our new AAV Data Hub, an open ...production and data analysis. Our first paper came out last year on sucrose supplementation to increase AAV2...AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending SfN2019, stop...

...blog.  We’ve been mostly working remotely for the last year to give our lab and shipping teams enough space...made their way onto our Slack workspace over the last year. Now, we’ve collected photos of our pets with... You will find five photos this way. At the very last photo, you will find a link to a form where if you...

...education resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR ...- JEI's future - getting more involved in the classroom by building curricula and developing one-on-one...

...unseasonably warm weather we had last weekend). We’ve spent the last week perusing the Deck The Lab contest...2019 Hope you’ve enjoyed the festivities in the last month!  ...

...Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology Plasmids Browse our CRISPR Guide Pages ...our CRISPR featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic...

...and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications...career advice collection. Embracing Serendipity Nicolas Mathis, who shared information on his open software...

...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

...Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your Research  ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and...

...behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants received sponsorship...Please do not include the fact that you are a classically trained ballerina who has performed at Lincoln... papers, awards, presentations, in .pdf format.  Last but not least, NETWORK! Networking is extremely ...Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...

...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

...! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to start...everyone’s favorite section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look...new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those...

...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...