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Showing: 501 - 520 of 757 results

  1. In Memoriam - The Origins of pBABE and Generations of Cancer Research

    Type
    Blog Post
    Published
    Sept. 29, 2017, 4:44 p.m.
    ...efficiencies to stand a chance of representing the complexity of cDNA libraries derived from tumors.  In addition... recombination that would lead to replication competent retrovirus.  When we arrived at the final vectors...

  2. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

  3. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...

  4. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...lab equipment on? This applies to water baths, computers, and more. Consolidate your autoclave runs. In...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to...

  6. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

  9. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian...

  10. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...

  12. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ... online calendar and can be accessed from your computer or smartphone. Evernote allows you to create notes...ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to adapt, likely...

  13. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications...

  15. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    .... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary.   3. “There are...

  17. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral CRISPR library systems described in companion papers in Science in December were developed as...colleagues, including David Sabatini and Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of ...

  18. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  19. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...
Showing: 501 - 520 of 757 results