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  1. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience...

  2. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...notes, since the conference, I think I may have finally grasped just how complicated DNA repair pathways...genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document...

  6. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...clusters of individual cells along with color coded nominated reference cell types.  (Adapted from Joung et ...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...dCas9 fused to the Activation Induced Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

  9. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly...

  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded...

  14. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

  15. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS...

  16. Plasmids 101: Environmental Plasmids

    Type
    Blog Post
    Published
    Jan. 31, 2017, 3:30 p.m.
    ...integrate into the host chromosome using DNA recombination systems. Some of these systems have been modified...One way to triumph over your neighbours is to eliminate the competition via the production of toxins and...

  17. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...Networking - Building Relationships for Success" webinar with Joanne Kamens via ASCB Check out more Career...

  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

  19. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...website, as an instructive step-by-step guide.  Finally, when writing your alt text, use all of this information... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...
Showing: 501 - 520 of 741 results