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TypeBlog PostPublishedJune 23, 2020, 1:15 p.m.... more broadly to accelerate COVID-19 research: Sinai Health System ...Essen University of North Carolina at Chapel Hill University of Illinois Chicago ...
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Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....Addgene ID number as well as the reference for its original publication. To help facilitate this process, ...also increases the visibility of both you and the original depositor. Our citations feature makes it so that... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be... be left on hold for an hour. When you finally speak to a human being, you may not even get a solution... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....are thus limited by its lower resolution. The combination of this method with superresolution microscopy... is so high, cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....than intensely competitive. Be curious and interdisciplinary. 3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m.... ligand from the Bryan Roth lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been...activation Targeting Further restricted to illuminated region Impacts all cells expressing receptors... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m.... reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m....at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked...coworkers were only a Slack message away. When it finally came time for me to present, I thought I would ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m.... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity. As SLs are also known to trigger seed germination of parasitic plants, this approach was also used... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....in “negative data” often dominate the early years of a project. Dissemination of the knowledge gained ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...for single and multi-color imaging experiments. Finally, what kind of system will you use to detect your... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....requested from Addgene many times as the technology disseminates throughout the research community. These popular...Takashi Yamamoto. “Addgene has made it possible to eliminate geographical restrictions in life science research... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m.... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....this quality. I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m.... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....beamsplitter, and a 495/30 nm emission filter. This combination results in a 2.6-fold increase in signal relative...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....will help get your experiments back on track. One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene ...