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Showing: 481 - 499 of 499 results

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An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

Published

Dec. 17, 2020, 1:30 p.m.

...the “elegans” methods outlined in this post – particularly the use of co-selection methods – could greatly...
RNA Interference in Plant Biology: New Tools for an Old Favorite

Type

Blog Post

Published

Oct. 27, 2020, 1:15 p.m.

...silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing...
Important Considerations When Using AAVs

Type

Blog Post

Published

June 13, 2017, 2:30 p.m.

...receptors (i.e. chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

Published

Dec. 15, 2016, 3:57 p.m.

...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...
Sequencing Options for CRISPR Genotyping

Type

Blog Post

Published

Oct. 4, 2016, 2:30 p.m.

...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

Published

May 28, 2024, 1:45 p.m.

...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Type

Blog Post

Published

March 21, 2024, 1:15 p.m.

...stable signal over extended periods, which is particularly beneficial when studying dynamic processes like...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Type

Blog Post

Published

June 28, 2018, 3:01 p.m.

...spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ...
Antibodies 101: Introduction to Antibodies

Type

Blog Post

Published

Jan. 19, 2021, 2:15 p.m.

... Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...
Which Fluorescence Microscopy Techniques is Best for Me?

Type

Blog Post

Published

Oct. 10, 2017, 1:57 p.m.

...tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

Published

July 11, 2017, 2:30 p.m.

... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

Published

April 25, 2017, 2:30 p.m.

...leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

Published

July 19, 2016, 2:30 p.m.

...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

Published

Nov. 6, 2018, 1:12 p.m.

...38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation in vitro where...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

Published

Sept. 10, 2020, 11:45 a.m.

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...
CRISPR 101: Cytosine and Adenine Base Editors

Type

Blog Post

Published

Aug. 6, 2020, 2:30 p.m.

...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...
Antibody Validation for Flow Cytometry

Type

Blog Post

Published

Sept. 10, 2024, 1:15 p.m.

...different providers for the same clone. This can be particularly useful when designing larger multicolour panels...
Delivery Methods for Generating iPSCs

Type

Blog Post

Published

April 17, 2018, 1:37 p.m.

...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent...
Technical Design of a Western Blot

Type

Blog Post

Published

Aug. 22, 2024, 1:15 p.m.

...important part of your western is your antibodies, particularly your primary antibodies. We always recommend...

Showing: 481 - 499 of 499 results

Results per page:

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

RNA Interference in Plant Biology: New Tools for an Old Favorite

Important Considerations When Using AAVs

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Sequencing Options for CRISPR Genotyping

Antibodies 101: Designing Your First Flow Panel

Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Antibodies 101: Introduction to Antibodies

Which Fluorescence Microscopy Techniques is Best for Me?

Custom CRISPR Screens & the Green Listed Software

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Special Delivery: Fluorophore Targeting for FRET Studies

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

CRISPR 101: Multiplex Expression of gRNAs

CRISPR 101: Cytosine and Adenine Base Editors

Antibody Validation for Flow Cytometry

Delivery Methods for Generating iPSCs

Technical Design of a Western Blot

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