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Showing: 461 - 480 of 738 results

  1. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression...

  2. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize...

  3. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making...

  4. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were...

  5. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ...

  6. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door...

  7. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Editing Consortium hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing...non-homologous end joining (Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: ...

  8. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics...

  9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

  10. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

  11. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...derived were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include...

  12. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for...

  13. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene...

  14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  18. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  19. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...
Showing: 461 - 480 of 738 results