...insert. Example, if you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert...

...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

...we wouldn’t necessarily have expected when we originally sent out the call for applications. Cervical ...caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls...

... the launch of our fast-growing ready-to-use recombinant antibodies collection, currently composed of ...the community to drive new science experiments. Finally, we were thrilled by how many people accessed our...

...2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...cells, deciphering the mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase...

...of the high fidelity version (despite what the original paper says). In their hands, BsaI-HF has produced...they hope others will do the same. “This isn’t the final version,” she said. “If we learn how to make the...

...adding plasmid) to make sure you don’t have contamination.  Also conduct a positive control (test a ... Melina Fan...

... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives...The afternoon program mixed things up with a combination of shorter, more narrowly focused lectures, interactive...

...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...adding Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now...

...forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity, which raises...Giving think tank event where we brought a multidisciplinary group of scientists together to draft a research...

..., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and... over-expressed, constitutively activated or inactivated the pathway. “The idea is that if you introduce...

...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

...different cell types, at different times, and in combination with different genes. Having CRISPR systems for...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...that you confirm the details and final experimental outcome in the original publication. As you develop and...

...than intensely competitive. Be curious and interdisciplinary.   3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people...

...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be... be left on hold for an hour. When you finally speak to a human being, you may not even get a solution...

...are thus limited by its lower resolution. The combination of this method with superresolution microscopy... is so high, cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity...

...Addgene ID number as well as the reference for its original publication. To help facilitate this process, ...also increases the visibility of both you and the original depositor. Our citations feature makes it so that...

... more broadly to accelerate COVID-19 research: Sinai Health System                                     ...Essen                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...

... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...