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Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

Published

Sept. 8, 2016, 2:30 p.m.

...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

Published

March 17, 2016, 2:30 p.m.

...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

Published

Oct. 6, 2015, 2:30 p.m.

...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...
The Importance of a Fun Workplace: Company Culture at Addgene

Type

Blog Post

Published

Sept. 3, 2015, 2:30 p.m.

...something fun is going on and to get there fast! Spread word of your plans by email, gong, evite, posters...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

Published

May 15, 2014, 3:33 p.m.

... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

Published

Aug. 14, 2018, 12:42 p.m.

...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

Published

July 25, 2019, 12:03 p.m.

...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

Published

July 23, 2019, 12:46 p.m.

...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

Published

April 11, 2017, 2:30 p.m.

... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...
Antibodies 101: Reading a Flow Plot

Type

Blog Post

Published

Feb. 6, 2024, 3:13 p.m.

... density of cells, while contour lines that are spread apart show a low density of cells (Figure 7). ...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

Published

April 7, 2016, 2:30 p.m.

...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

Published

Oct. 27, 2015, 3 p.m.

... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

Published

Oct. 1, 2015, 2:30 p.m.

...collaboration and the exchange of ideas. The rapid spread of optogenetics throughout the neuroscience field...
Plasmids 101: Control Plasmids

Type

Blog Post

Published

April 9, 2015, 3:29 p.m.

...or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

Published

April 3, 2014, 8:05 p.m.

... phosphoglycerate kinase gene. Constitutive Widespread expression, but may vary by cell type. Tends ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

Published

March 5, 2019, 1:08 p.m.

...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...
Plasmids 101: Biotinylation

Type

Blog Post

Published

Nov. 15, 2018, 1:50 p.m.

... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

Published

Aug. 23, 2018, 12:05 p.m.

...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

Published

April 9, 2019, 1:13 p.m.

...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

Published

Nov. 16, 2021, 2:15 p.m.

...Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

Showing: 421 - 440 of 448 results

Results per page:

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Tips from the Repository Trenches: Using Barcodes to Track Samples

The Importance of a Fun Workplace: Company Culture at Addgene

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Neuronal labeling with Spaghetti Monster

Save Time with Transient Plant Leaf Transformations

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Fluorescent Proteins 101: Aptamer Fluorophores

Antibodies 101: Reading a Flow Plot

Getting the Most from Your Lentiviral Transduction

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: Control Plasmids

Plasmids 101: The Promoter Region – Let's Go!

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Plasmids 101: Biotinylation

Fluorescent Proteins 101: When GFP lets you down

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Antibodies 101: Introduction to Immunofluorescence

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