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Showing: 421 - 440 of 645 results

  1. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...answer to the space problem. Instead of imaging smaller, what if we just made the sample bigger? In ExM...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C....

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...parties, and the company at large, by polling and informal communications we made a decision to try out a...the other hand if the journal club occurs with a small technical team (single lab or team), a deep technical...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...properly, it is unstable and may only work for a small number of qPCR runs before a new one is needed. ...and the droplet generation oil are moved through small channels to create a water-in-oil emulsion containing...

  8. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...can be used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...

  9. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...higher Tm give more efficient recombination. We normally use a Tm between 47-55 °C for the homologous region...be very helpful to confirm primer design.  Use minimal template DNA Use 1 ng of PCR template DNA to minimize...

  10. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...amount and type of information you want to convey. Small tidbits and fast/fun facts are easy and engaging... be oversaturated. Transition from academic to informal writing can difficult.  Photography Medium...

  12. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells could repopulate... systems used in both papers are analogous to mammalian viral vectors in that the vectors are replication-deficient...

  15. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... treat a bacterial infection with a lower than optimal drug concentration. Drug resistance is not a black...patient infection. Limiting the use of antibiotics in animal farming is already being recognized as an important...

  17. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...requiring rapid maturation and prolonged imaging with minimal background.   Find these C. elegans mScarlet plasmids...18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 kDa) and are...

  19. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-negative  Acinetobacter...campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1 ...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...
Showing: 421 - 440 of 645 results