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Showing: 421 - 440 of 672 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

  4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

  7. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...determinants of prime editing efficiency such as the length of the RTT overhang, the GC content of the primer... pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...entire time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual...they are following along. This will not only re-engage you with those who sought out your poster, but ...

  11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to ...technologies currently available, you now face the challenge of trying to figure out what system might work...

  12. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...

  13. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...models in species that were previously difficult to engineer, like rats. If you have tools for creating knockout...

  15. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

  18. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

  19. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...
Showing: 421 - 440 of 672 results