Skip to main content
Addgene
Showing: 401 - 420 of 634 results

  2. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded into Nanoblades...transduction for most cell types they tested. Interested in using Nanoblades? Plasmids are available from...

  3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology park...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27). Joined...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... are all wired up and expressing your opsin of interest, it’s time to get to the fun stuff: the experiments...Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory in opioid...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...selected to preferentially target your cell type of interest. Viral pseudotyping is also a powerful tool that... optimize them for targeting your cell type of interest. For in vivo imaging applications, the ability...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors to VP1,...into a compartment encapsulating the protein of interest (Figure 3).   Figure 3: Schematic representation...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  14. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “...would be complete without the inclusion of some interesting case studies, and Zimmer provides several. In...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...active in various gram-negative bacterial hosts of interest, including within mixed communities and into species...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

  17. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

  18. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Let's Go! How to Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid...Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest Analysis We also introduced guides...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... answers to such questions, Addgene is deeply interested in pursuing them. We’ve recently revamped our...can help others as much as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest...
Showing: 401 - 420 of 634 results