...(cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library by inserting...Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to...region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence...sequence. When viruses produced from this plasmid pool are injected into an animal with cell-type-specific Cre... do these AAVs transduce the CNS and PNS? Both plasmids infect the PNS or the CNS much better than the...

...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the...detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound...difficult-to-study RNA-modifying enzymes, such as the RNA demethylases FTO and ALKBH5. Their ease of production have...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

...Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain multiple ...the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used this information...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...

...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile...upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...

...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...

... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... Surface labeling is particularly useful for classifying and sorting cells by identifying lineage markers...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live cells... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies...

...Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality for...infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk for other chronic...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

...problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine base editors (CBEs...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in ourselves... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

...and beyond. Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors...biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our fast-...knowledge and research opportunities. I have spent the last 9 months quietly learning about Addgene’s past and...

...AAV receptor (not strictly required)2 Human fibroblast growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1...pseudotypes are described with two numbers separated by a slash: the first number refers to the genome, and the ..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Request Browse all in-stock AAV preps Browse all AAV plasmids ...

...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

...for at least a couple of decades. Green is the classic color, but fluorescent proteins are available in...in 2011 and then in another Nature Methods paper last year. The key to their bacterial phytochome-derived... could see deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable...the researchers described in Nature Communications last year, “The capability to control spectral properties...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team reported...

...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...