...internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...

...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription... is less than that of electroporation, but an advantage is that chemical transformations are generally...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

.... This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer ..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...

...technology potential enabled by CRISPR-transposons has yet to be fully realized. Many thanks to our guest...applications - as mutagenesis tools for genetic studies (e.g. using mariner), as a method of tagmentation for next-gen...

...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over...

...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...

...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...useful for viral reproduction, and for causing contagious infection; however this would not be great for...

...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...transferred is commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial...

...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...

...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less...

...-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl substitutions...

...can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with respect... at 1 paper/week will be useful. Check out the hashtag #365papers on Twitter for inspiration and accountability...

...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...of current methodologies. This section sets the stage for why your funded proposal will address these ...

...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...who are interested in such careers should take advantage of their academic environment and attend seminars...

...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...

...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...