...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here... The sense of trust and comfort I had with my teammates acted as my safety net and trampoline all at once... I really believe that the culture at Addgene cultivates trust between coworkers and that trust then translated...

... may appear to be binding at particular genomic sites when in fact they are just non-specific or indirectly... MNase. Once MNase has been localized to target sites, the nuclease is briefly activated to digest the... an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets,...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

...carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in ...collection of empty backbones that have been designed, tested, and published by academic scientists. To help ...a mutated strain or a mutated version of YGOI to test the different phenotypes of the specific mutations...

...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...opsonization of IgG and IgM antibodies on a pathogen activates the immune system's classical complement pathway.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky...time to completely digest the template DNA. Note—templates must have been isolated from methylation competent...

... can’t safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase...I got good at this despite the fact that my lab mates thought this was hilarious.  Birth of the baby gel...

...labs have websites listing alumi and it is easy to use publication records, university websites, ResearchGate... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...be run in parallel and provide serotype data in minutes and the scripts  can be easily customized to detect...

...avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged ... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

...have recently received a grant from the National Institutes of Health that will address this problem.The ...animals may be variable among animals and bleed dates. The development of monoclonal antibody (mAb) technology... represent the official views of the National Institutes of Health.     References and Resources References...

...building that someone from a different company had tested positive for COVID-19 (it turned out to be an unfounded...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...

...of heredity were thought of as everything from parasites, to symbionts, to genes and the labels applied... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

...Avidin and its derivatives have four biotin-binding sites per molecule so, theoretically, saturated avidin...were unable to cleave TEV within the protein we tested, it may be possible with other fusion proteins ...bacteria significantly enhance protein folding (tested using real science). Make a stock solution of 2.5...

...is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences...molecular pathways, I found myself curious about other routes – In terms of both types and topics of work. A ...rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination to be ...

...scicomm group project on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer...industry. Along this road, science communication graduates are often well-equipped. Many institutions are...explores risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications...

...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...in a competitive transplant assay in the mouse intestine, demonstrating its utility in long-term tracking...

...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and transduction...host of other AAV serotypes; it will be exciting to test this approach with different capsids. Most importantly...

... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

...CRISPR/Cas system, Cas9 is guided to its target sites with the aid of two RNAs: the crRNA which defines... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist of repeated...