... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...

...since it is impossible to check all possible combinations of biology and equipment. Because the performance...(Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

...s ability to process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive...processing, i.e. tRNAs or Csy4 cleavage sites. This eliminates bulk from crRNA expression plasmids resulting...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...treatment process. The authors went on to show that combination treatment with multiple drugs reveals even smaller...heterogeneity to drug discovery and the development of combination therapies.         References 1. Hyo-eun, C. ...

...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...cloning, waiting, and repeating before you can finally dive deep into generating the data you’re excited...cost $155.25 in reagents and consumables today. Finally, this doesn’t account for the most valuable component...

... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

...or awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies... Addgene ID for their deposited plasmids in the final journal publication or an updated preprint, further...

...played enormous roles in the development and dissemination of fluorescent protein tools. This year, we’...creates a painful sensation. The dorsal horn of the spinal cord is the first site of sensory input from our... the dual virus expression in CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi...

...ligation assay (PLA). The clever combination of FUNCAT and PLA PLA was originally published by the Söderberg...16590564. PubMed Central PMCID: PMC222790. 6. Buxbaum, Adina R., Bin Wu, and Robert H. Singer. "Single β-actin...

...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...

...time). Other illumination strategies, such as 2-photon-excitation, selective plane illumination, TIRF or ...experiments on a single day on a stable system. A final piece of advice is to measure the excitation power...

... the protein or structure of interest. For a C-terminal tag, as shown in the example above, we inserted...would be transcribed and translated. For an N-terminally tagged protein, we used the same strategy, inserting...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional...

...yellow, or magenta, depending on the hairpin combination bringing the total number of individual colors...Adding an additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase...15! They also envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very...

...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...inconsistencies across geographical regions When we originally started gathering this information, many assumed...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...

...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...members all say the same thing and one panel member dominates the discussion? Or maybe the one that goes way...front of a crowd. The moderator will then develop a final list of questions for the actual event, based on...

...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...

...researchers to use. Although we haven’t yet made our final target decisions, we intend to focus on motor neuron... a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces are ...equipment for new uses. We’ll probably never use the Illumina GAIIx we recently acquired  for sequencing again...

... incident, and the sterilization of women in marginalized communities have created a legacy of distrust...included taking extra coursework and attending seminars in bioethics, public health, science policy, and...advantage of their academic environment and attend seminars, symposia, or courses in social, health or policy-related...

...PCR product that comes from amplification of the original plasmid which can over-inflate results. A packing...vectors carrying fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our...into a target cell’s genome. This approach can discriminate multiple integration events, reducing the possibility...