...to a variety of careers outside of academia. She has had previous graduate students and postdocs go on...interviewed Addgene depositors who are at various stages in their careers to get advice on choosing a lab...

...amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...

...logical pattern across the poster. This style has the advantage of being different and potentially more catchy...to your audience. With a poster, you have the advantage of being able to verbally communicate your work...and acknowledgements sections are inserted. The advantage of this style is that it will display your data...

...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone... to create engineered mouse models at embryonic stages, and the pros and cons of different genome editing...mice are engineered during the embryonic stem cell stage using one of several techniques. The longest standing...

...platform. During the conference If the conference has a social media policy, this should be on their website...conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for the ...Check out the conference website for any relevant hashtags and handles to help you find other scientists ... share their research on social media. Use the hashtag if you are live-tweeting any sessions that the ...

...’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...

... is easy, after all, who working in a laboratory has never faced an odd situation with experiments? Our...major challenge when we started was creating a LEGO stage that resembles a real laboratory. It only took one...

...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...

..., another method to create point mutations, also has a strict editing window about 15 nt upstream of the...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. ...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...

...The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is ...Spring in Lower Geyser Basin. They used a mini-metagenomics method using microfluidic parallelization to...

...their advantage should they decide to produce virus on their own.Harvard researcher Connie Cepko has been...

...writing, etc. Also, it is unlikely that a mentor has the only relevant expertise and perspective for your...been doing something all wrong or that a reagent has not been convincingly demonstrated to work for your... be needed to achieve your intended next career stage and create a timeline for achieving these goals....

... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...using viral vectors! One of the most significant advantages of viral delivery is the potential for long-term...results in calcium assays. There’s another crucial advantage: tissue-specific targeting. By employing various...

...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll... organizations or cities may require a higher percentage of the decontaminate to be used for BSL-2 waste...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate...

... Prize in Chemistry for his work. Since then PCR has become a standard and essential practice in molecular...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

... interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...they can share on their resumes have a definite advantage in our process. We see candidates who have done...

...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...get more done. There are also a host of other advantages to practicing effective delegation skills: ...

...regions of the gene is also useful in case the gene has a weird mutation that you didn’t know about (which...and is a testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months... the bands on your gel and get an idea of the percentage of cells in the population that have an edit....