...and Protein Modifications in Cells and Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org...need a little help to be useful. Mainly because, well, antibodies are kind of hard to see. To solve this...imaging instruments or X-ray film and lends itself well to assays like western blots and ELISAs. Chromogenic... like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et...throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988). If some of those lysines are in...doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino...epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038...

...gRNA technology have enhanced targeting efficacy as well as the unnecessary stress of worrying about the ...evolved ways to prevent degradation of important cellular RNA molecules with post translational modifications...deoxynucleotides. A partially DNA gRNA is surprisingly well-tolerated by the Cas9 protein and can increase on-target...slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some...transfection efficiency and even track localization in cellular experiments. There are many suitable dyes to choose...enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi....Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https:/...

...proteins in cells. Homeostatic regulation of protein abundance is essential for normal cell function, and...one particular protein in neurons or in any other cell type. However, we cannot distinguish new from old...facilitate the visualization of specific proteins in cells. Tagged protein can easily be overexpressed from...techniques to follow translation in living single cells. However, before CRISPR-Cas9 these techniques were...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ... turnover of specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger... how small molecule fluorophores can be used for cell labeling Check out other protein tagging strategies...

...depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study the spike variants...protein, called HexoPro, was developed by Jason McLellan’s lab. This version of the spike protein was developed... investigate how the virus interacts with human cells. Addgene’s mammalian target plasmids express these...scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation), from Addgene...for SARS-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their search, they found...packaging) and contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids...Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based design of prefusion-...

...is non-pathogenic, well-studied and inexpensive. In addition, a complete set of well-annotated deletion...contain methionine. (This allows cells carrying nonfunctional as well as functional MET genes to grow.... or students clone similar sequences from a less-well studied organism into overexpression plasmids. Students...synthesis in S. cerevisiae (Figure 1) occurs through a well-characterized pathway with multiple enzymatic steps... is available at our Investigations in Molecular Cell Biology Website. Many thanks to our guest blogger...right) has taught courses in biochemistry, molecular cell biology and genetics at Boston College. She is particularly...

...editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652.e29. https...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...type of edit, genomic context, delivery strategy, cell type, and goal of the experiment. Now, onto the ...nicking of the unedited strand and encourage the cell to use the edited strand as a template during DNA...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A...

...to give yourself plenty of time when writing a fellowship proposal. Yes, yes, I hear you saying “We know...research (either because of expertise, equipment, or excellence of the institution). Likewise, if you’re staying.... Become a science communicator More and more fellowship applications are asking for a 200-300 word explanation...explanation of your research for non-scientists. Most fellowships are funded by the general public (either through...may make your perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically...assisting me with applications for much larger fellowships. Also, if you were awarded money to carry out...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...

...currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the Rockefeller University.  His interests...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. ...Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674. doi: 10.1016/j.cell.2015.06.058. Pubmed PMID: 26189679...Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...

... genetic loci within a single cell. One might imagine transfecting cells with both the nuclease-positive...Perhaps most importantly, most Cas9 orthologs are less well characterized than the conventionally used SpCas9... both to lower relative nuclease efficiencies as well as to more stringent targeting rules that result...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as ...reliance on orthogonal Cas9 species. Within a single cell, full-length guides targeting one set of genetic...nanoparticles as drug delivery systems. She is also a NASA Fellow studying biosignature preservation in Mars analogue...knockin mice for genome editing and cancer modeling." Cell 159.2 (2014): 440-455. PubMed PMID: 25263330. PubMed...

...produced by a single B cell. To create a monoclonal antibody cell line, myeloma cells are fused with activated...activated B cells from the spleen of mice injected with the antigen of interest. The immortalized cells, called...distributing over 3,000 antibodies and associated hybridoma cell lines. The DSHB was created by the National Institute... individually isolated and expanded. The clonal cells are screened to identify hybridoma lines, each producing...

... infected cells to go undetected by the immune system in a novel way, by shielding host cell surface proteins... itself to escape vesicles within host cells and turn the cell into a veritable virus factory.  Their ... sets off.  The virus works its way into immune cells in ways that aren’t fully understood. Much of the... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...have information or another resource to add, please tell us about it in the comments. ...

...indicated that inntags IT5, IT6, and IT10 expressed well in cells and did not form undesirable aggregates. Using...tags. Impressively, IT6-GFP did not reduce yeast cell size or viability compared to untagged GFP overexpression...multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6 fusions...and HA tags had especially detrimental effects on cell size and growth rate, indicating that they compromised... replace our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags...correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID...

...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ... chemiluminescent proteins, like luciferases, as well as fluorescent ones, like Green Fluorescent Protein...green glimmer, Shimomura collected many, many jellyfish specimens from Puget Sound in the Pacific Ocean...he was able to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin... could be used to measure gene transcription, as well as to track protein localization. And as it turns..., another part of the fluorescent protein puzzle fell into place, as they, taken alongside the insights...

...bodies that will generally segregate with the cell pellet during purification procedures. Even if you produce...codons at random and expect your sequence to express well in any organism. So what are the evolutionary constraints...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different...Tuller et al. found that translation efficiency is well correlated with codon bias in both E. coli and S...’t necessarily mean the gene is going to express well. If you do get good expression, you should also ...mismatched translation rates and potential effects on cell growth, even hosts producing alternative tRNAs may...a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290. PubMed...

...Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016/j.cell.2013.01.038 Guy J, Gan J, ...deliver the precise amount of protein to each brain cell to restore physiological levels. Much research is...repair a gene in a living animal across multiple cell types using a mouse model for a human neurological... that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling the root...Resource Center. Researchers can also find links to cell line repositories including patient-derived iPSCs...Modifications in Human-Induced Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep...

...Generating a no-fuss genetic knockout  Knock-out cells or animals are engineered by introducing Cas9-mediated... which cuts at NRN PAMs with high efficiency, as well as NYN, but with a slightly lower efficiency. Information...transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9 along...study essential genes whose knockout would cause cell lethality. The Krüppel associated box (KRAB) is ...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating...activating target genes in a variety of species and cell types can be found on Addgene’s website. ...optimized for expression in different species and cell types including (but not limited to) bacteria, yeast...

...currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the Rockefeller University.  His interests...optogenetics, ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber...refine and optimize your stimulation parameters as well. Inhibition of neuronal activation is a little ... remote contextual memories. This study is an excellent example in which the authors started with a brain... research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321...Dynamics of retrieval strategies for remote memories. Cell. 2011;147(3):678-89. Pubmed PMID: 22179551. Pubmed...

... want to make sure your cells have first reached an appropriate density. Cells in the exponential growth...collecting dead cells, and, conversely, you cannot induce too early as there are not enough cells in the culture... purposes, these E. coli strains are not usually well suited for recombinant protein expression. Many ...host protein, using up valuable resources in the cell thus leading to slowed growth. Additionally, some... promoter. Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak...such as TB or 2XYT is optimal because of the high cell-density they support; however, minimal media supplemented...

... disease, cell type or drug treatment can browse through screens performed in relevant cell lines or conditions... currently houses over 890 CRISPR screens in 629 cell lines, each manually curated and annotated from ...feasibility and power of CRISPR-based library screens in cell lines from humans and other species. By accurately... screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. Each screen ...screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system, sgRNA library and...example phenotype screens with read-outs other than cell viability. The following video provides an in-depth...

...knock out, etc. – CRISPR can be used to generate cell line or animal models which have the precise disease...Since CRISPR has been adapted for use in primary cells, many tissue types, and across species, it enables... phenotype. CRISPR can also be used to generate cell line knock-ins of patient mutations which are variants...CRISPR has revolutionized the generation of knockout cell lines which has made this control step extremely...in a cancerous background and treat the parental cell line and the X knockout with your drug(s). If a ...research field! References and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug...the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit Disease Out of the...