...across the USA during this time have revealed that young scientists are aware of, and are engaged in, these...change the landscape facing all scientists, both young and established, as they embark on or continue their...along with progress over the past two years give young academic scientists a foot in the door, but we will...

..., which is critically important, especially for young researchers. It was a really nice incubator for ...academic positions and poor grant situation. So, young researchers need to publish good publications in...transforming conventional research. So, I hope my younger colleagues may be able to develop new technologies...

..., Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing DNA Methylation in...Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation and activation...Mendenhall EM, Williamson KE, Reyon D, Zou JY, Ram O, Joung JK, Bernstein BE (2013) Locus-specific editing of...

...experiments move closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique...specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks to our guest blogger Søren Hough...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities...

...Heart AAV1, AAV8, AAV9 Liver AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 RPE (Retinal Pigment Epithelium...PMC4678200.  Kohara, K., Pignatelli, M., Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa...R.J.O., Mori, T., Finke, S., Conzelmann, K.-K., Young, J.A., and Callaway, E.M. (2007). Monosynaptic Restriction...

...advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar...set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament even dyed with...

... field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically...dramatically and reducing off-target effects. Other young scientists discussed new CRISPR delivery systems...

...wanted to run and play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him...Kurt has been running on behalf of AJ Gonzales, a young boy suffering from FOP, raising money for NORD for...

...calcineurin is required for virulence in pathogenic fungi, it is an attractive drug target. Calcineurin reporters...as a drug-screening platform to identify new antifungals that target calcineurin. Current calcineurin ...

... KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan W-M, Ip JD, Chu AW-H, Zhou J, Lung DC, Kok K-H, To ...

...role in Cas9 activity and efficiency (Sander and Joung, 2014). Thus it is essential to identify the best...//doi.org/10.3389/fcell.2018.00054 Sander JD, Joung JK (2014) CRISPR-Cas systems for editing, regulating...

...Ahringer Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related.... Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS...

...modified version of the Python program described by Joung et al. 2017, which we hope to make available as ...:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt...

...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308. Additional...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...public on the implications of their work. Johnny Kung is the Director of New Initiatives for the Personal...

...GeCKO v2) 123,411 20 bp 600–1200 ng Solid Joung et al. Nat Protoc. 2017 CRISPR Knockout Human..., plus a 24 bp barcode 250–1,000 ng Solid Joung et al. Nat Protoc. 2017 with modifications Screening...

...retired! Conversely, cells younger than the 3rd – 4th passage are too young and should not be used for...

...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative... 86 (2016): 14-25. PubMed PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial ...

...model organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If...codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19...

... as anthocyanin production, or disease, such as fungal growth. Healthy plants produce lots of seeds. More...mean more chances to recover a transformant. Use young plants: If you transform early, your plants will...