...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

... Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High Gene Expression...High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects...

...any gene that they could be used by people with little bioengineering expertise? “This is the next step...improve activator design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error...

...social media, not from their institution), is how little agency they have in their salaries and benefits...suffice it to say, salaries are showing surprisingly little correlation with cost of living at the location...

...was ready for a change of scenery. She moved to Seattle and continued working for Palladian Partners remotely...also tired of the contracting life where you do a little bit of work for one client and then switch to doing...

...vector and Murine LoxP vector.   Turn it off for a little while Transient suppression of expression is faster... Lenti expression vector. Turn it on for just a little while Yes, there’s a CRISPR for that, too! CRISPR...

...The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence...make a precise amino acid change, there is very little flexibility in the location of the cut and subsequent...

... in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated...databases to the production of validated sensors in as little as one week (Pardee et al, 2016). Using toehold...

...excited for this next step. But wait, now you feel a little panicked. You’ve never had an industry interview...day.  This whole process may seem foreign and a little overwhelming, but here is what I’ve found helpful...

...gkt520 Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand... . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan...

...the protease cleavage sequence. The tag is then shuttled to the proteasome due to the presence of the degron...tagged protein. In this event, the degron domain shuttles the entire tagged protein to the proteasome for...

...that satisfy all requirements. To alleviate this bottleneck, we began building CrispyCrunch in July 2018 ...Although this practice is generally advised, there is little detail published on how to do it best. Scientists...

..., creating a presentation for 8th graders was a little intimidating. Too simplistic and I would insult...section was arguably the most important, and it was a little worrisome for me since bioethics is an inherently...

...feeling optimistic for the next tournament). Minus a little rain towards the end, it was another successful.... I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes fun needs to be had...

...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized... work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene...

...should be able to find your mutant from screening as little as 8-10 pups. The level of fluorescence you saw...day you have your F0 generation (barring life’s little accidents and stuff ups). This is a huge improvement...

...people until the day of the AMA. This made it a little difficult to promote the AMA on social media beforehand...relationships. I also like Twitter because I can get a little opinionated and, while it can be anonymous, I can...

...Animal husbandry of diverse species is a real bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea...This is a known stem cell and regeneration model. Little bits of hydra can grow back into a full hydra and...

...Leaving a tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of ...editor, etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy ...

...accordingly in case you have questions or need a little assistance at some point during the procedure. ...with questions and concerns is invaluable. With a little thought you can build meaningful relationships ...