... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...

...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global...

... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

...as a free event! Organizing a science conference for the public How does one organize a public scientific... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... graduate student organization Science in the News (SITN), we recently sponsored a first-time event called...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...opportunities to improve our communication and organizational skills provide great training for a number ...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm....

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...          Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...

...body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent...pathways, it is possible to map the internal organization of a cell. This mapping can be done by using antibodies...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ... proteins” which are known to be a part of an organelle or involved in intracellular transport pathways...for commonly used markers for major mammalian organelles and transport pathways. It is best, though not...

...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of blog... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation... additional resources and information about organizations promoting reproducibility in this booklet (which...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...within a published manuscript. Luckily, many organizations have been working to provide tools that make...

...another successful event! Addgene company culture At Addgene we organically developed the philosophy of...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...

...away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3...this Friday will combine all of these. Addgenies have been organizing Halloween festivities for years – searching...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...

... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...

... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. A protein-tagging system for signal amplification...

...nonprofit or U.S. governmental research organizations, and organizations that require the H-1B employee to ...entity, or nonprofit research organizations or governmental research organizations. Since there is no numerical... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...Additionally, since most universities and non-profit organizations do not have the April 1 H-1B queue process or... or quota like industry, these organizations can be a good choice for individuals needing sponsorship.... in your field. Membership in a professional organization – this is not referring to one that you can ...program self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert...

...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...context defined by ligand, cell type, and promoter, organizing the expression of functional groups of genes ...sections of the current NURSA website. NURSA and Addgene The great progress that has been made in the NR...literature and acquiring them in a timely manner. Addgene represents an excellent solution to the problem...

...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...Additional Resources Read other Career Posts on the Addgene Blog! ...

...them paralyzing an organization. I think these are things that I can bring to Addgene -- that comfort with...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on Addgene.org Try the... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...

...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection Learn...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...