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We narrowed to 128 results for: jun

Showing: 21 - 40 of 128 results

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 May ...

  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389/fcell.2022.880107. KRaION...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...dimer catastrophe hypothesis. Mol Microbiol. 1993 Jun;8(6):1031-8. doi: 10.1111/j.1365-2958.1993.tb01648...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...multiplexing vectors at Addgene! Multiplexing in plants Qi-Jun Chen Lab Golden Gate/Gibson Assembly Multiplexing...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...bfloGFPa1 and bfloGFPc1. Bomati et al., Sci Rep 2014 Jun 27;4:5469. MitoTimer plasmids to report mitochondrial...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...effects of the injunction nationwide and, at exactly one month following the injunction, 59% of all postdocs...salary increase prior to the injunction still won’t after the injunction (see below). Of the institutions... known before the injunction, are raising salaries, even in light of the injunction. In addition, PIs ...per year. This post discusses how a nationwide injunction against the FLSA is affecting universities' decisions...below). However, on November 22nd a preliminary injunction against the FLSA updates was granted nationwide...salaries. What are institutions doing after the injunction? We are particularly interested in whether institutions...institutions planning to raise salaries prior to the injunction are still honoring these plans. We are now collecting...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...at the ligation junction. Thus, all four bases at either side of the ligation junction were represented...containing one or more mismatches near the ligation junction with high efficiency (11, 21). Ligases are thought...discrimination at the upstream side of the ligation junction (the base pair providing the 3 ́-OH terminus to...Watson-Crick and mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions...

  15. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can...channels and even between many cells (through gap junction proteins which connect the cytoplasm of two cells...side should grow a head and a tail, and when gap junction-mediated electrical communication is briefly inhibited..., Néstor J., et al. "Long-range neural and gap junction protein-mediated cues control polarity during ...

  16. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ... can see that those in teaching roles (such as adjuncts) and graduate and undergraduate students are exempted...this have not been received. Future actions for junior scientists and for academia Changes are afoot for... that tenured professors/mid-career scientists/junior faculty will all be most/least likely to have to...Research. He is interested in advocating for and with junior scientists, to help them navigate the scientific...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus ... amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also...Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500...

  18. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ... IIS restriction enzymes and careful choice of junction sequences guided by experimentally-derived DNA...overhang observations, accurate “high-fidelity” junction sets for both the 12- and 24-fragment versions...lacZ cassette were designed and synthesized. In conjunction with BsaI-HFv2 (NEB #R3733), re-engineered to...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog characterized...Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...ultimately leading to the formation of double Holliday junctions (dHJs). dHJs are four-stranded branched structures...resolved via cleavage in one of two ways - each junction resolution could happen on the crossing strand...orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing strand and the other...
Showing: 21 - 40 of 128 results