Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
Type
Blog Post
Published
March 1, 2016, 3:30 p.m.
... to the standard restriction/ligation, Gateway, or Golden Gate cloning methods. Whether it is for routine...actually work by significantly different methods. Golden Gate cloning does result in the seamless joining of...location and NOT in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional...