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  1. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read ...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...extra elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...
Showing: 21 - 23 of 23 results