...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

...(YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection markers...

...virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and...lacunosum moleculare were better resolved with smFP_FLAG than GFP.   3. Enhanced ability to distinguish...

.... Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down menu...

...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate...

...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

...there are many types of epitope tags (eg. HA, MYC, Flag), they can be deleterious and cause aberrant protein...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision...synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. ...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...

...by Paix et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered...digests of PCR products used to identify a single 2xFLAG knock-in by direct screening. The center images...mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes into other, non-linked loci; pha-1(e2123...

...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...

...reach zooxanthellae, the coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000... chromoproteins could be used for mimicry and camouflage. An anemone’s red colored tentacles resemble ...

...everywhere, but resources are not. Through its flagship program, Instrumental Access, Seeding Labs partners...

...After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's focus...

... or PreScission), epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX...

...thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins...

...money to sustain a startup, he went to work for Flagship Ventures – a local Cambridge biotech venture capital...

...like-minded science communicators? Attend the annual flagship meetings of AAAS, WCSJ or ECSJ. These meeting ...

...FPs as well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in ...

... currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone Aiptasia...