Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
Type
Blog Post
Published
March 19, 2019, 1:31 p.m.
...control of CRISPR-Cas9 by CASANOVA
Article contributed by Cary Valley
Listen to the anti-CRISPR podcast ...podcast segment
Anti-CRISPR (Acr) proteins potently inhibit type II CRISPR systems including the popular S. ... cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and pUC57... of protein coexpression. By introducing a single plasmid containing both GOIs, expression levels are ... them a rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a ...domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., ... protein AcrIIA4. In the dark, AcrIIA4 inhibits CRISPR activity. With the addition of blue light, the...