A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
Type
Blog Post
Published
Nov. 2, 2021, 1:15 p.m.
...information.
Optimize titer for the DIO/FLEx vector
Once you have determined the optimal injection parameters...begin to optimize the DIO/FLEx vector. This step is essentially the same as what was used to optimize the ...the causes of off-target expression and ways to optimize experiments to minimize it.
Overview of DIO/FLEx...frt-STOP-frt (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the recombinase...created with BioRender.com
Strategies for optimizing expression
So now that we know spontaneous recombination...promoter with in vivo experiments to determine the optimal/minimal injection titer and volume needed to achieve... injection titers and volumes to determine the optimal injection parameters needed to transduce your cells...