...Cas9 and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find more plasmids...36 kb in size, which does not integrate into the host genome, removing the risk of oncogenicity or genotoxicity...disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An alternative...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...

... or reverse transcriptase enzymes, inhibition of host cell proteins, and modifications to the pegRNA. ...recently used a CRISPRi screen to identify additional host factors impacting prime editing and found the protein...functionally reconstituting coding sequences for a range of applications, including CRISPR activation by ...simple yet valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting...

...particularly retroviral vectors because they target a broader range of cells and their integration into the genome...immunogenic proteins generated Stable integration into host genome and stable expression of the transgene ...protein (VSV-G) that improves the stability and broadens the cellular tropism of the viral particles produced...determine their concentration. Three methods are broadly used for virus titration: the p24 ELISA dosing ...

...variety of backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering...federal or state level some professional societies host “Hill Days” where they train and bring researchers...can inform your move into science policy. Read broadly about the historical roots of science policy to...

...large cargo capacity, minimal cytotoxicity, and broad host-tropism. These tools open up many powerful avenues...differ from each other by their light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM ...iLID micro; Figure 1). With this great affinity range, these LIDs have been shown to be useful tools for...quantified. This strategy relies on the current range of mitochondrially targeted photoactivatable and...reduced system leakiness and increased the dynamic range, resulting in more user-friendly and tunable tools...et al 2015[2]). The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to ...variant of PSmOrange, a photoswitchable fluorescent protein which transitions from emitting orange (λ= 565nm...