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Showing: 21 - 29 of 29 results
  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...captures the streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut...target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized with streptavidin...that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm target binding...
  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...streptavidin-binding peptide (SBP). The addition of biotin disrupts this interaction and thus triggers a synchronous...
  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Generation Toolbox! Starting with libraries and a biotinylated (for purification purposes) protein target of...process, displayed sybodies that bind to the biotinylated target protein can be recovered for the subsequent...plate to capture Myc-tagged sybodies. Then, the biotinylated target protein is added and after a few washes...
  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...bi-directionality. For predominately anterograde tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent...Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer for single-...
  5. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...Combo Consists of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine...
  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins...
  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...need more signal, another strategy is to use a biotinylated secondary antibody then label that with fluorescently-conjugated...
Showing: 21 - 29 of 29 results