SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
Type
Blog Post
Published
May 5, 2020, 1:15 p.m.
...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...sequence to bind to the sample and upon binding, gets activated. This activation results in a trans cleavage...detection.
Highlights: Cas13a is not catalytically active in the presence of two or more mismatches in the...complementary region in the target sequence and this activity results in the trans cleavage of the ssDNA-FQ ... given fluorescently labelled substrate, gets activated and performs non-specific collateral cleavage....cleavage by the enzyme and instead relies on its activity on the target regions thus making it an affinity-based...the Sashital Lab, Iowa State University. She is actively interested in science communication and enabling...