... cells that contain a specific gene survive. The rest do not grow. While there are many ways to design...You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture...some now have plasmids that contain your gene of interest, but others will uptake plasmid backbones that...antibiotic resistance cassette along with your gene of interest. In these cases, antibiotic selection will select... if the plasmid backbone contains your gene of interest. Auxotrophy Besides antibiotic selection, you ... select for a successful uptake of your DNA of interest by taking advantage of bacterial characteristics...by recombination sites. To introduce a gene of interest into the destination plasmid, recombination sites...

...comprehensive pre-compiled downloads and via a full-featured REST Web Service API. Both options are fully open access...CRISPR results for any gene of interest. In addition, users interested in a particular disease, cell type...literature for a drug, disease, or condition of interest using a simple keyword search. For example, searching...entire CRISPR database for particular screens of interest.   Figure 3: Each publication featured ...access and available without any licensing restrictions.    As CRISPR technology continues to develop and...

...solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum...fusing a fluorescent protein to your protein of interest, you instead fuse it to the synthetic SunTag scaffold...

...and we’d love to know what you think. As with the rest of our services, you can bet that we’ll be continually...

...system. The effector is placed downstream of the rest of the SPARC components and expression is controlled...used to specifically manipulate a cell type of interest in an organism, all cells of that type are affected...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a...

...CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed by three of the biggest... weren't quite sure what to expect in terms of interest, but visitors to the poster were impressed to ...edge science in increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing...

...from the rest of our panel. Lastly, we want to detect the expression of our protein of interest: CD45. ...It also means we should exclude APC-Cy7 from the rest of our panel. Besides some background cell death...through genetic modification. As your protein of interest will only occur in combination with that reporter...antibody-fluorophore combinations for different markers of interest, you can separately detect these markers in your...

...just go to sessions that you find interesting and not go to the rest.” Having an on-site conference canceled...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...aspect and I could plan more with regard to my interests.” Use the chat box and upvote If the thought of...

... with the virus. Then the viral particles do the rest! Downstream expression or integration resulting ...constructed viral vector containing the cargo of interest is then typically transfected along with viral...

...This variant exhibits high contrast between its resting state and its calcium-bound state, which was achieved...

... as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique...

... now you don’t have to worry about operating the rest of the car as well. Similarly, cell free expression...keeping. Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on...student at MIT in Biological Engineering and is interested in developing synthetic biology based tools for...

...FPbase data in their own projects and applications, a REST API allows programmatic retrieval of the majority...that serve to further characterize a protein of interest (and we very much appreciate even those small ...

...of curated plasmids from all over the world. The rest of this piece will describe plexWell™ in more detail...

...the PCR product Isolate your PCR product from the rest of the PCR reaction using a kit, such as the QIAquick...assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for...plasmid of interest. You can use similar processes to add overhangs to your insert of interest for Gibson...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...discussed in our restriction cloning post. However, even more than with standard restriction cloning, it is...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...itself are very similar to those outlined in our restriction cloning post with a few quirks specific to the...

...Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How... careful not to go too far. Is the assignee interested in this task? Don’t give out tasks – where possible...

...and screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This...

... 180 regions to another or from the brain to the rest of the body. Therefore, by simply looking at how...

...the meeting and then communicate outcomes to the rest of their team. Here's a template for project meetings...

...and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...