...these plasmids. This is because in 2024 certain export control exceptions were made by the US Bureau of...majority of the countries we ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular...   For researchers in countries with applicable export control exceptions for rabies and VSV-G genes, ... and we strive to keep our information updated, export controls and regulations are complex and may change...

...Problems with FRET kinase reporters Difficult to Multiplex: FRET reporters are comprised of two fluorescent...phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded...between the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...single-cell kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex...alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a...multiplexed together. Challenging  to Design: FRET reporter activity depends on both the distance and the ...

...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...- or reward-related behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into...Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian...contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists...heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance, and sometimes...Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression...with a resolution of less than 100 µm, the ARG reporters could be successfully visualized in vivo using...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit...

...exist for all Addgene plasmids! In an effort to support standardized persistent identification of research...Research Resource identifiers (RRIDs) which are supported by SciCrunch. Other well known PID formats are... the journal’s specific author guidelines for reporting your materials. If you deposit a plasmid with ...plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats. Acknowledgments...

...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are...Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized ...panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate ...Image from Diessl et al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial...

... español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más ...

...customized fluorescent reporters can be challenging enough, but incorporating multiple reporters into a single ... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs... used to study transport characteristics of various Golgi and plasma membrane reporters as well as secretory... protein transport. Franck Perez’s lab has developed a whole collection of hook and reporter proteins ...pyralis) reporter plasmids for use in high-throughput screening of pre-mRNA splicing. This reporter system...widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been...

...the pSer-incorporated polypeptide. These three components were shown to effectively incorporate phosphoserine...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified...considerations, it is important to determine how much phosphorserine has actually been incorporated into the full-length...different levels of incorporation due to differences in stability of the p-Ser incorporated protein as well...determine the stoichiometry of phospho-incorporated to non-phospho-incorporated proteins purified from the system...pSer-incorporated to non-phosphorylated protein. Verification of site-specific pSer incorporation can ...

... facilitate transport: retrograde transport relies on dynein, while anterograde transport relies on kinesin...take advantage of existing transport pathways in neurons. Anterograde transport is typically used for the... transmitter synthesis. Retrograde transport is used for transport of endocytosed material or molecules... impaired, and only few reports exist about dye molecules that are transported from one neuron to another...There are several reports of both retrograde and anterograde intracellular transport of some AAV serotypes...including dendritic and axonal extensions and, most importantly, any connections the neurons make to other distant...for various forms of retrograde and anterograde transport suggest that there are several parallel mechanisms...

... whether respondents saw them as not important to very important. In addition, we also explored the barriers...”. The study also showed that It is important that an ELN supports all types of data, which enables researchers...electronic ones? It is important to know in which ways your data can be exported or printed from the ELN...project. Can you meet the ELN support team? We cannot stress enough how important this is when deploying an...only 46% could be reproduced. In a recent Nature report from researchers at Amgen, it was stated that only...through all saved data and automated writing of reports. To be able to understand the situation in depth... is likely to require on-going maintenance and support.” Besides that, academic institutions tend to think...

...conjugated with a reporter or tag that can then be detected.  Typically, the reporter is an enzyme such...indirect and sandwich ELISA the reporter signal is directly proportional to the level of antigen in the...whereas in a competitive ELISA the reporter signal is inversely proportional to the level of antigen in the...dish and incubate it with a reporter conjugated primary antibody. The reporter reacts with a provided substrate...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection, users... sandwich and competitive. In a direct ELISA a reporter enzyme conjugated antibody binds to antigen coated...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to ...

...you think there are four important times to measure that growth rate, you report the results of all four... registered reports, the gold standard for testing hypotheses Finally, Registered Reports combine all ...Reproducibility Project: Cancer Biology, where reported methods may not include how many tests were conducted...attention on the results instead of the methods. Unreported flexibility and questionable research practices...result after conducting a replication study. An important difference between the original setting and the...genetic variation, or different handling can all be important.  If we eliminate the problems above: unclear ...research finding we can be more certain that some important, previously unknown variable actually makes a ...

... to cleave and activate a reporter. The detectable signal from this reporter can be measured and quantified...and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...variants, Cas13, and Cas12a, to develop simple, portable, and inexpensive platforms to reliably detect ...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both SHERLOCK and... SHERLOCK- Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in...molecules. In SHERLOCK a quenched fluorescent ssRNA reporter is added to the reaction. Cleavage of the quenchable...

... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...removal of GFP from the TRICK reporter. Delayed translation is known to be important in development, and Halstead...proteins to allow translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the...GFP protein that binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP...translation, both GFP and RFP are bound to the reporter, causing it to appear yellow. The act of translation...of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization signal...copies of the FP-coat protein fusions to their reporter mRNAs. Using translation inhibitors cycloheximide...

...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...genes as reporters and some that repress instead of activate transcription. The HIS3 reporter system (...sequence (UAS) of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether...co-expressed in a yeast strain that also contains a reporter gene cloned downstream of a promoter containing...referred to as the “bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI...Gal4 activator, bacterial LexA DBD and the lacZ reporter gene (3, 4), there are now many variations, including...to have Y2H systems that avoid transcriptional reporters altogether; In the split-luciferase system, intein-mediated...

...cuts to important research funding.  So when I saw that there was going to be a march in support of science...the rain, about 4,000 supporters of science showed up to march. We had full support of local law enforcement...proved to be the perfect opportunity to cement in my children's heads the importance of science and positively...seems to break everyday. Many researchers and supporters of science marched to advocate for scientist ...for science, and numerous other reasons. It is important to remember that we, scientists and non-scientists...°W – Vanessa Sung Hundreds of scientists and supporters in Montreal took to the streets on April 22 to...Albany, Rep. Paul Tonko also came and spoke in support of science - he's one of the few members of Congress...

...has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed to be packaged... The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...couples NLuc to fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins...host cells. The fluorescent moiety of the BRET reporter was used to sort successfully infected/expressing...NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals without exogenous excitation...

... to report mitochondrial turnover Mitochondrial health, transport and turnover are important features...genes, reporters, or other markers into a target genome. They did this by selecting the important elements... be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter using...usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s ...s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial degradation...mitochondria in the networks marks the differences in import of newly synthesized (green) protein and organelles...channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked channelrhodopsin with a spectral...

...mentor Your manager should support you, just like you are supporting your report(s). This means that you ...unique skills and strengths (you will likely have reports with different levels of experience), find out ...how to prioritize but I  just added the “most important” tasks my schedule. This was bad for 2 reasons...tasks before I was a manager! Second, my direct report was not engaged because I only left him/her with...expectations, answer any questions, and (most importantly) decide on a timeline. My experience is that ...you’re delegating a recurring task to a seasoned report, there should be questions! Remember that delegating...develop your team members by giving them new opportunities. I still need to check myself sometimes to ...