....  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796. PubMed Central PMCID: PMC52876. 2. Novel approach...

...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic resistance...

...tumours via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w.... what you'll find in this post: CRISPR-Csm: A novel RNA targeting system Heat-inducible lentiviral ...ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system by: Susanna Stroik The Doudna...

...Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role for a nuclear...

...interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735. PubMed.   ...papers, published in Molecular Systems Biology in November, Voigt and Alex Nielsen describe what’s possible...

...GECIs. Frontiers in molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal...

...e3647. doi: 10.1371/journal.pone.0003647. Epub 2008 Nov 5. PubMed. Efficient design and assembly of custom...Gate and genome engineering In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-...Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(...

...Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413...prior with the death of Jesse Gelsinger in an adenovirus-based gene therapy clinical trial gone horribly...destructive inflammatory immune response. At the time, adenoviral vectors were the most commonly used viral vectors...Virus (AAV) While other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known to transduce...

...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...

... RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central...dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, but without...

...Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171. Efficient isolation...University. The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of ...

...CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse Geneticist's...

...was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...ways to circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences... from IGI!) Synthetic S. pyogenes Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed...2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding specificity...remaining variants (VQR, EQR, and VRER) recognize novel PAM sequences (shown above). The VQR, EQR, and VRER... Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically...

...reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015 Nov 5;5:16277. PubMed PMID: 26538064. PubMed Central ...

..., Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1;450(7166):56-62. PubMed. A technicolour approach...

... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected...

.... coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible new tools for epitope...biological concepts and employ them in creative and innovative ways. The principle of protein multimerization...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...the first round of translation in living cells A novel technique developed in the labs of Jeffrey Chao,... 10.1073/pnas.1420024112. Epub 2015 Feb 23. A novel dCas9 activator capable of unprecedented activation...synergistic effect on target gene activation. The novel dCas9-VPR activator is capable of enhancing target...Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides a novel strategy for assaying the dynamics of different ...

...and two-way ANOVAs ANOVA tests can compare either one (one-way ANOVA) or two (two-way ANOVA) independent... form of ANOVA. Isn’t that what a two-way ANOVA test is you might ask? Not exactly! A MANOVA test assesses...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...variation that needs to be accounted for. ANOVA tests ANOVA tests are essentially t-tests with room for...one-way ANOVA can test either the effect of temperature or humidity on crop production.   MANOVA The MANOVA... and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent variables...the “C” that differentiates these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance...

...in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...fact: There are 57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     ...will discuss the gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The components...cell types. AAV also requires some back up from adenovirus genes – E4, E2a, and VA – which are supplied ... within their viral elements. Gene expression Adenovirus is a linear, dsDNA virus naturally. It has ITRs... you wish to deliver to your target cells. In adenovirus, the ITRs serve as a type of origin of replication...of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid components...