...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low... 59.3 (2018): 1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling...

...sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...The pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep...types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of interest...

...: 25184501. PubMed Central PMCID: PMC4262738. 3. EGFP gRNA (BRDN0000561167) (Plasmid #80034) and BRAF ...off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith...

...FLAG, EGFP, etc) on your insert. Use the drop-down menu to indicate whether the tag is on the N or C terminus...

...Terminus 79629 (N) and 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635...NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting...Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They.... The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal... (< 100 bp) and large (up to 120 kb) deletions in N. benthamiana and Arabidopsis. The authors found that...

...cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated...increase the FRET efficiency. Creating fusions at the N- and C- termini of your protein of interest The most...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein...to describe the different parts of the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 ...We have encountered several proteins for which the N- and C-terminus of the POI are necessary for its native...fluorescent protein into the POI can work well since the N- and C-terminal residues of the fluorescent protein...monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the...