...blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., ...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

...of our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018...

...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ...

...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

... derived from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis...

...) Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a...Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a...

...facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to...

...played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden...

...and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (...

...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6),...

...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038...

....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids...

...depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for ...

...-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...

...through Addgene. For example, you could enter "Feng Zhang (Addgene plasmid #52961)".   The vector type ...

...PMC2629495. Zingg, B., Chou, X., Zhang, Z., Mesik, L., Liang, F., Tao, H.W., and Zhang, L.I. (2017). AAV-mediated... S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn,...

...Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N. Y., Pickel....2022.05.003 Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan...Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A., Wade, K., He, J., ...Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., ...

...domain and a mostly c-terminal nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of ... of varying fusion protein expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371...