...need. For example, as shown in this study [3], hippocampal neurons are activated when the mouse is placed...expression, thus showing successful inhibition of hippocampal neurons. Initial monitoring Second, this may ...associated with a visual or auditory cue. The hippocampus has long been known to be responsible for mediating...the function of specific subregions within the hippocampus were only recently studied in detail thanks to...

...culminated in the current President holding and campaigning on many views that don't line up with the scientific...at the University of Colorado, Anschutz Medical Campus.  He studies viruses including HIV and is interested...And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use the same... at the University of Colorado Anschutz Medical Campus in Aurora, CO where she studies the development...

...Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor...Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep ...

...strange to be using a micropipette and gloves at a campsite. And it was exciting – one night while trying ...picnic table before dark, a bison wandered into our campsite. We had to abandon our samples and move farther... Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia...

...Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid...730825. https://doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj CM, Chuapoco MR, Chen X, Goeden N...Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V., Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian...Ravindra Kumar, S., Arokiaraj, C. M., Plattner, V. M., Campos, L. J., Mich, J. K., Monet, D., Ngo, V., Ding, ...Scherrer, J., Winchester, J., Blackburn, R. J., Campos, L. J., Man, K. N. M., Sun, J., Chen, X., Lefevre...

...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."...

...Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus...).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 ...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

... we’re here to help with our Suggest a Plasmid Campaign. How to suggest a plasmid The scientists at Addgene...reach out to will end up depositing, we hope this campaign will expand the repository in ways that will be...

...optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically...

...Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically...

... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus of ... at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski...

...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...

... disease for which there is currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab...see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated silencing...

...biosensor, GCaMP, and click through to the plasmid search results: More than 200 different GCaMP containing...

...Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is ...currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would rather...

...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Are you...and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. His interests...

...brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2 serotypes in...visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal neurons which are known to be notoriously...

...AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...